期刊
THERIOGENOLOGY
卷 53, 期 1, 页码 11-20出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0093-691X(99)00236-8
关键词
in vitro production; oocyte competence; embryo; atresia; cow
The current knowledge is reviewed concerning correlations between follicular development in the cow and the competence of matured oocytes to develop into an embryo following IVF and IVC. At the follicular size of 3 mm, some oocytes become competent and the proportion of competent oocytes does not increase during development up to 7 mm. The proportion of competent oocytes increases greatly in follicles >8 mm in both untreated and gonadotropin-stimulated cows. The competence of in vitro-matured oocytes hem these large follicles is lower than the competence of in vivo-matured oocytes. These observations lead to the following concept. Oocytes have acquired an intrinsic capacity to develop into an embryo after IVM-IVF-IVC at the follicular stage of 3 mm, but require an additional prematuration to express this competence. In vivo, this prematuration occurs during preovulatory development before the occurrence of the LH surge. In follicles of 3-7 mm, a low level of atresia appears to improve the in vitro competence of oocytes which may act via a prematuration-like effect. A thorough understanding, however, of the effect of atresia and other factors on the competence of this highly heterogeneous oocyte population is still missing. Two routes to improve the embryo yield in ovum pick-up (OPU) practice are discussed. (C) 1999 by Elsevier Science Inc.
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