期刊
PEPTIDES
卷 21, 期 1, 页码 73-80出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0196-9781(99)00176-X
关键词
proglucagon; radioimmunoassay; GLP-2; GLP-1; DPP-IV
By using radioimmunoassays toward the cDNA-predicted amino acid sequence of human glucagon-like peptide-2, a peptide was isolated from extracts of human ileum. By mass spectrometry and Edman sequencing, this peptide was identified as human proglucagon 126-158. High-performance liquid chromatography analyses indicated that a similar immunoreactive peptide (iGLP-2) was present in human plasma. Human plasma concentrations of iGLP-2 were elevated 3- to 4-fold at 1 to 2 h after ingestion of 800 to 1200 kcal meals. (C) 2000 Elsevier Science Inc. All rights reserved.
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