期刊
GENE THERAPY
卷 7, 期 1, 页码 53-60出版社
STOCKTON PRESS
DOI: 10.1038/sj.gt.3301044
关键词
Epstein-Barr virus-based plasmid vector; episomal vector; polyamidoamine dendrimer; gene therapy; herpes simplex virus thymidine kinase; Ewing's sarcoma
This study demonstrates in vivo effectiveness of a nonviral vector system, Epstein-Barr virus (EBV)-based plasmid vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBV-based vector is a plasmid vector containing EBV nuclear antigen I (EBNA1) gene and oriP from EBV genome. HSV1 tk gene was transferred into Ewing's sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based plasmid vector pSES.Tk, or a conventional plasmid vector, pS.Tk. Cells transfected with pSES. Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared with those transfected with pS.Tk/dendrimer. Intratumoral injection of pSES.Tk/dendrimer but not pS.Tk/dendrimer drastically suppressed the growth of tumors which had generated from A4573 or Huh7 hepatocellular carcinoma (HCC) cells inoculated into severe combined immunodeficiency (SCID) mice. The treatment with pSES.Tk/dendrimer also resulted in significant prolongation of survival of the mice implanted with A4573. These results suggest that the EBV/polyplex system could be useful for in vivo suicide gene therapy of cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据