期刊
CHEMICAL RESEARCH IN TOXICOLOGY
卷 13, 期 9, 页码 846-852出版社
AMER CHEMICAL SOC
DOI: 10.1021/tx0000771
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资金
- NCI NIH HHS [R01 CA65878] Funding Source: Medline
- NIGMS NIH HHS [GM19388-02] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA065878] Funding Source: NIH RePORTER
Analysis of the reaction between 2'-deoxyadenosine and 13-hydroperoxylinoleic acid by liquid chromatography/constant neutral loss mass spectrometry revealed the presence of two major products (adducts A and B). Adduct A was shown to be a mixture of two isomers (A(1) and A(2)) that each decomposed with the loss of water to form adduct B. The mass spectral characteristics of adduct B were consistent with the substituted 1,N-6-etheno-2'-deoxyadensoine adduct 1 -[3-(2'-deoxy-beta-D-erythro- pentafuranosyl)-3H-imidazo[2,1-i]purin-7-yl]heptan-2 -one. Adducts A(1), A(2), and B were formed when 2'-deoxyadenosine was treated with synthetic 4-oxo-2-nonenal, which suggested that it was formed by the breakdown of 13-hydroperoxylinoleic acid. A substantial increase in the rate of formation of adducts A(1), A(2), and B was observed when 13-hydroperoxylinoleic acid and 2'-deoxyadenosine were incubated in the presence of Fe-II. Thus, 4-oxo-2-nonenal was most likely formed by a homolytic process. Although adducts A(1), A(2), and B were formed in the reaction between 4-hydroxy-2-nonenal and 2'-deoxyadenosine, a number of additional products were observed. This suggested that 4-hydroxy-2-nonenal was not a precursor in the formation of 4-oxo-2-nonenal from 13-hydroperoxylinoleic acid. This study has provided additional evidence which shows that 4-oxo-2-nonenal is a major product of lipid peroxidation and that it reacts efficiently with DNA to form substituted etheno adducts.
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