期刊
ULTRASOUND IN MEDICINE AND BIOLOGY
卷 26, 期 7, 页码 1169-1175出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0301-5629(00)00260-X
关键词
sonoporation; bioeffects of ultrasound; therapeutic ultrasound
Lethal sonoporation and reparable sonoporation were observed in Jurkat lymphocytes in suspension with the addition of varying amounts of Optison(R), a commercially available bubble-based contrast agent. For given ultrasound (US) exposure conditions (spatial peak-pressure amplitude of 0.2 MPa, duty cycle 10% and 2-MHz frequency), sonoporation was directly related to the bubble-to-cell ratio (in a range from 0 to 230), It was found that the nearest bubble-cell spacing was also related to the occurrence frequency of bioeffects, A constant bubble-to-cell ratio often provided very different results for two different initial cell concentrations (200,000 cells/mL and 600,000 cells/mL), with the higher cell concentration generally exhibiting higher levels of sonoporation. In contrast, a constant bubble-to-cell spacing provided similar results between the two initial cell concentrations, The frequency of reparable and lethal sonoporation was seen to decay as the inverse-cube power of the nearest bubble-cell spacing. Significant reparable sonoporation was observed at a bubble-cell spacing that was 10 mum larger than the minimum spacing at which significant lethal sonoporation was observed. Preliminary analysis also suggests the possibility of a step-wise increase in lethal sonoporation as spacing decreases; further experiment is needed. (C) 2000 World Federation for Ultrasound in Medicine & Biology.
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