期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 84-6, 期 -, 页码 561-576出版社
HUMANA PRESS INC
DOI: 10.1385/ABAB:84-86:1-9:561
关键词
softwood; pretreatment; acid hydrolysis; enzymatic hydrolysis; ethanol
Whole tree chips obtained from softwood forest thinnings were pretreated via single- and two-stage dilute-sulfuric acid pretreatment. Whole-free chips were impregnated with dilute sulfuric acid and steam treated in a 4-L steam explosion react-or. In single-stage pretreatment, wood chips were treated using a wide range of severity. In two-stage pretreatment, the first stage was carried out at: low severity to maximize hemicellulose recovery. Solubilized sugars were recovered from the first-stage prehydrolysate by washing with water. Tn the second stage, water-insoluble solids from first-stage prehydrolysate were impregnated with dilute sulfuric acid, then steam treated at more severe conditions to hydrolyze a portion of the remaining cellulose to glucose and to improve the enzyme digestibility. The total sugar yields obtained after enzymatic hydrolysis of two-stage dilute acid-pretreated samples were compared with sugar yields from single-stage pretreatment. The overall sugar yield from two-stage dilute-acid pretreatment was approx 10% higher, and the net enzyme requirement was reduced by about 50%. Simultaneous saccharification and fermentation using an adapted Saccharomyces cerevisiae yeast strain further improved cellulose conversion yield and lowered the enzyme requirement.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据