期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 84-6, 期 -, 页码 743-749出版社
HUMANA PRESS INC
DOI: 10.1385/ABAB:84-86:1-9:743
关键词
Aspergillus niger; cellobiase; solid-state fermentation
Aspergillus niger NRRL3 was cultivated in a moist wheat bran and ground corncob solid medium supplemented with inorganic minerals for the production of cellobiase (beta-1,4 -glucosidase, EC 3.2.1.21). With this method, A. niger NRRL3 was able to produce a high concentration of cellobiase (215 IU/g of solid substrate) after 96 h of incubation. Temperature and moisture content affected final cellobiase titers. The best conditions for cellobiase production from solid substrate by A. niger NRRL3 were determined to be 70% moisture and 35 degrees C.
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