Kiwifruits (Actinidia deliciosa) transformed with a Vitis stilbene synthase gene produce piceid (resveratrol-glucoside)

A stilbene synthase gene was isolated from three Vitis spp. (V. vinifera. V. labrusca and V. riparia). These genes were placed under the control of the cauliflower mosaic virus 35S promoter and introduced into kiwifruit (Actinidia deliciosa) plants by; Agrobacterium-mediated gene transfer. The introduced gene(s) were expressed and piceid (resveratrolglucoside) rather than resveratrol was produced in the Leaves of the transformants. Resveratrol produced by the action of the integrated gene(s) seems to he metabolized into piceid by an endogenous glycosyl transferase. Among the transformants obtained, the highest piceid content in the young leaves was 182 mu g/g fresh weight. Although these transformants did not show resistance against Botrytis cinerea, which causes gray-mould disease, the fruits may have some beneficial effects on human health.