期刊
NEUROSCIENCE RESEARCH
卷 36, 期 3, 页码 245-250出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0168-0102(99)00131-5
关键词
calcium; circadian rhythm; excitatory amino acids; rat; suprachiasmatic nucleus
We have previously reported that spontaneous release of excitatory amino acids (aspartate and glutamate) show remarkable circadian rhythms in the organotypic slice culture of rat suprachiasmatic nucleus (SCN). Here we showed effects of extracellular Ca2+ removal and of L-trans-pyrrolidine-2,4-dicarboxylic acid, a glutamate/aspartate uptake inhibitor on the circadian release of excitatory amino acids in the SCN culture. Amino acids were measured by high-performance-liquid-chromatography. Removal of extracellular Ca2+ exerted no effect on the spontaneous release of the excitatory amino acids, while it blocked high K+-evoked release of the amino acids. Neither the period nor the amplitude of the spontaneous circadian release of amino acids in Ca2+-free medium was different from those in the Ca2+-containing medium. On the other hand, L-trans-pyrrolidine-2,4-dicarboxylic acid increased the excitatory amino acid levels without affecting the amplitude of excitatory amino acid rhythms. These results indicated that the circadian release of excitatory amino acids in the SCN is Ca2+-independent and L-trans-pyrrolidine-2,4-dicarboxylic acid- insensitive. Therefore, Ca2+-dependent chemical synaptic transmission may not be involved in the circadian rhythm generation in the SCN. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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