期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 16, 期 8-9, 页码 829-834出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1008987103701
关键词
alcoholic fermentation; ethanol; hemicellulose; hydrolysate; recombinant Escherichia coli KO11; sugar cane bagasse
Escherichia coli KO11, carrying the ethanol pathway genes pde (pyruvate decarboxylase) and adh (alcohol dehydrogenase) from Zymomonas mobilis integrated into its chromosome, has the ability to metabolize pentoses and hexoses to ethanol, both in synthetic medium and in hemicellulosic hydrolysates. In the fermentation of sugar mixtures simulating hemicellulose hydrolysate sugar composition (10.0 g of glucose/1 and 40.0 g of xylose/1) and supplemented with tryptone and yeast extract, recombinant bacteria produced 24.58 g of ethanol/1, equivalent to 96.4% of the maximum theoretical yield. Corn steep powder (CSP), a byproduct of the corn starch-processing industry, was used to replace tryptone and yeast extract. At a concentration of 12.5 g/1, it was able to support the fermentation of glucose (80.0 g/1) to ethanol, with both ethanol yield and volumetric productivity comparable to those obtained with fermentation media containing tryptone and yeast extract. Hemicellulose hydrolysate of sugar cane bagasse supplemented with tryptone and yeast extract was also readily fermented to ethanol within 48 h, and ethanol yield achieved 91.5% of the theoretical maximum conversion efficiency. However, fermentation of bagasse hydrolysate supplemented with 12.5 g of CSP/1 took twice as long to complete.
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