Phylogenetic relationships of the genus Paramecium inferred from small subunit rRNA gene sequences

The genus Paramecium includes species that are well known and very common in freshwater environments. Species of Paramecium are morphologically divided into two distinct groups: the bursaria subgroup (foot-shaped) and the aurelia subgroup (cigar-shaped). Their placement within the class Oligohymenophorea has been supported by the analysis of the small subunit rRNA gene sequence of P. tetraurelia. To confirm the stability of this placement and to resolve relationships within the genus, small subunit rRNA gene sequences of P, bursaria, P. calkinsi, P. duboscqui, P, jenningsi, P, nephridiatum, P, primaurelia, and P, polycaryum were determined and aligned. Trees constructed using distance-matrix, maximum-likelihood, and maximum-parsimony methods all depicted the genus as a monophyletic group, clustering with the other oligohymenophorean taxa, Within the Paramecium clade, P. bursaria branches basal to the other species, although the remaining species of the morphologically defined bursaria subgroup do not group with P. bursaria, nor do they form a monophyletic subgroup. However, the species of the aurelia subgroup are closely related and strongly supported as a monophyletic group. (C) 2000 Academic Press.