期刊
APPLIED MAGNETIC RESONANCE
卷 21, 期 3-4, 页码 411-422出版社
SPRINGER WIEN
DOI: 10.1007/BF03162417
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资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM036232] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM036232-15] Funding Source: Medline
A new variant of lipoxygenases, one containing manganese instead of iron, is characterized by electron paramagnetic resonance (EPR) at two frequencies. In the manganous state (S-e = 5/2), maganese lipoxygenase (MnLO) yields very broad X-band (9.2 GHz) EPR signals, extending over about 800 mT. In contrast, at W-band (94 GHz), the signal is much simplified, consisting, of nested transitions centered near the free electron g-value. Computer simulation has been employed to derive estimates of the zero-field splittings for MnLO, with data from these two EPR frequencies, The general features of both X- and W-band spectra are fit, first, by simulations with S-e = 5/2, but no nuclear hyperfine splitting. The simulations are then refined by inclusion of the hyperfine splitting. On the basis of the simulations, the ranges of zero-field splitting parameters are D = +0.07 to +0.10 cm, and E/D = 0.13 to 0.23. Comparison of the value of D for MnLO with that of other manganese-containing proteins suggests that MnLO has three N-ligands to the metal center and O-ligands in the remainder of 6 coordination positions. The coordination environment of MnLO is similar to that in iron lipoxygenases.
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