期刊
MICROBIOLOGY
卷 70, 期 1, 页码 73-78出版社
MAIK NAUKA/INTERPERIODICA
DOI: 10.1023/A:1004849022417
关键词
nitrogen fixation; nitrogenase genes; nifH; PCR; oligonucleotide primers
类别
Based on the analysis of the nifH gene nucleotide sequences from GenBank, a system of primers was developed that makes it possible to obtain 370- and 470-bp PCR fragments of the nifH gene of nitrogen-fixing bacteria and archaea. The effectiveness of the proposed system for revealing the presence of nifH genes was demonstrated by PCR on the DNA isolated from nitrogen-fixing prokaryotes for which the primary structure of these genes is known and which belong to different taxonomic groups. nifH sequences of nitrogen-fixing prokaryotes of the genera Xanthobacter; Beijerinckia, and Methanosarcina, for which the capacity for nitrogen fixation was demonstrated earlier, but no data existed on the nucleotide composition of these genes, were determined and deposited in GenBank.
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