Response of pollen germination and tube growth to cadmium with special reference to low concentration exposure

Cadmium is one of the most important heavy metal pollutants highly hazardous to plants. Pollen is considered to be more sensitive to pollutants than are vegetative parts of the plants. Five herb species were tested for responses in pollen germination and tube growth to Cd exposure in vitro. Pollen germination of all the species was inhibited at Cd concentrations of 2.51 mug/mL and higher, and tube growth was inhibited at concentrations of 1.58 mug/ml and higher. Cadmium, at low concentrations, stimulated pollen tube growth. The pollen response to Cd stress exhibited interspecies differences. Vicia angustifolia and V. tetrasperma were sensitive to Cd, and were inhibited in either pollen germination or tube growth by Cd at 0.01 mug/mL. Plantago depressa was less sensitive; pollen germination and tube growth were not inhibited until the Cd concentration reached 2.51 and 1.58 mug/mL, respectively, and its tube growth displayed two stimulatory peaks; the one that appeared at 1.00 mug/mL showed the highest tube length in all species tested. These results suggest that Cd, even at low concentrations, may adversely affect plant reproduction by inhibiting pollen germination and tube growth. (C) 2001 Academic Press.