期刊
MYCOPATHOLOGIA
卷 151, 期 1, 页码 35-39出版社
SPRINGER
DOI: 10.1023/A:1010953319092
关键词
cost-effective; isolates; media; Nomuraea rileyi; Spodoptera litura
类别
Cost-effective and rapid multiplication of Nomuraea rileyi is reported. The spore yields in semi-synthetic media were comparable or significantly higher to the standard medium. Maltose and peptone, carbon and nitrogen sources could be effectively replaced with 2% barley extract and 1% soybean extract respectively. However, replacement of yeast extract with dry yeast resulted in lower spore yields. Sporulation of the fungus multiplied on solid substrate was possible only when the bags used had a 0.2 mum filter to facilitate passive exchange of sterile air. A high spore yield of 2.8 x 10(9)/g of substrate was realized on crushed sorghum.
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