期刊
NEOPLASIA
卷 3, 期 1, 页码 4-9出版社
NATURE AMERICA INC
DOI: 10.1038/sj.neo.7900132
关键词
MARKL1; beta-catenin; microarray; expression profile; hepatocellular carcinoma
类别
Activation of the Wnt-signaling pathway is known to play a crucial role in carcinogenesis of various human organs including the colon, liver, prostate, and endometrium, To investigate the mechanisms underlying hepatocellular carcinogenesis, we attempted to identify genes regulated by beta -catenin/Tcf complex in a human hepatoma cell line, HepG2, in which an activated form of beta -catenin is expressed, By means of cDNA microarray, we isolated a novel human gene, termed MARKL1 (MAP/microtubule affinity-regulating kinase-like 1), whose expression was downregulated in response to decreased Tcf/LEF1 activity, The transcript expressed in liver consisted of 3529 nucleotides that contained an open reading frame of 2256 nucleotides, encoding 752 amino acids homologous to human MARKS (MAP/ microtubule affinity-regulating kinase 3), Expression levels of MARKL1 were markedly elevated in eight of nine HCCs in which nuclear accumulation of beta -catenin was observed, which may suggest that MARKL1 plays some role in hepatocellular carcinogenesis.
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