期刊
MICROBIAL PATHOGENESIS
卷 30, 期 1, 页码 19-28出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1006/mpat.2000.0406
关键词
human cerebral microvessel endothelium; E. coli; Citrobacter; invasion; transcytosis
资金
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS026310] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P50AG005142] Funding Source: NIH RePORTER
- NIA NIH HHS [P50 AG05142] Funding Source: Medline
- NINDS NIH HHS [R01-NS 26310] Funding Source: Medline
- PHS HHS [R01-A1 47225] Funding Source: Medline
Most cases of neonatal bacterial meningitis develop as a result of a hematogenous spread, but it is not clear how circulating bacteria cross the blood-brain barrier. Attempts to answer these questions have been hampered by the lack of a reliable model of the human blood-brain barrier. Human brain microvascular endothelial cells (HBMEC) were isolated and transfected with a pBR322 based plasmid containing simian virus 40 large T antigen (SV40-LT). The transfected HBMEC exhibited similar brain endothelial cell characteristics as the primary HBMEC, i.e. gamma glutamyl transpeptidase and a high transendothelial electrical resistance. Escherischia coli and Citrobacter spp, two important Gram-negative bacilli causing neonatal meningitis, were found to transcytose across primary and transfected HBMEC, without affecting the integrity of the monolayer. In addition, E. coli and C. freundii invaded transfected HBMEC as shown previously with primary HBMEC. We conclude that E. coli and C. freundii are able to invade and transcytose HBMEC and these bacterial-HBMEC interactions are similar between primary and transfected HBMEC. Therefore, our transfected HBMEC should be useful for studying pathogenesis of CNS infections. (C) 2001 Academic Press.
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