期刊
LAB ON A CHIP
卷 1, 期 2, 页码 102-107出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b109031n
关键词
-
类别
资金
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG001399] Funding Source: NIH RePORTER
- NHGRI NIH HHS [HG01399] Funding Source: Medline
A fully integrated genomic analysis microsystem including microfabricated heaters, temperature sensors, and PCR chambers directly connected to capillary electrophoretic separation channels has been constructed. Valves and hydrophobic vents provide controlled and sensorless sample positioning and immobilization into 200 nL PCR chambers. The use of microfabricated heating and temperature sensing elements improves the heating and cooling rates for the PCR reaction to 20 degreesC s(-1). The amplified PCR product, labeled on-column with an intercalating fluorescent dye, is injected into the gel-filled capillary for electrophoretic analysis. Successful sex determination using a multiplex PCR reaction from human genomic DNA is demonstrated in less than 15 min. This device is an important step toward a microfabricated genomic microprocessor for use in forensics and point-of-care molecular medical diagnostics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据