期刊
CHEMBIOCHEM
卷 20, 期 1, 页码 20-28出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201800394
关键词
biocatalysis; cascade reactions; enzyme fusion; enzymes; linkers
资金
- European Union Project ROBOX under the EUs Horizon 2020 Programme Research and Innovation actions [635734, H2020-LEIT BIO-2014-1]
One approach to bringing enzymes together for multienzyme biocatalysis is genetic fusion. This enables the production of multifunctional enzymes that can be used for whole-cell biotransformations or for in vitro (cascade) reactions. In some cases and in some aspects, such as expression and conversions, the fused enzymes outperform a combination of the individual enzymes. In contrast, some enzyme fusions are greatly compromised in activity and/or expression. In this Minireview, we give an overview of studies on fusions between two or more enzymes that were used for biocatalytic applications, with a focus on oxidative enzymes. Typically, the enzymes are paired to facilitate cofactor recycling or cosubstrate supply. In addition, different linker designs are briefly discussed. Although enzyme fusion is a promising tool for some biocatalytic applications, future studies could benefit from integrating the findings of previous studies in order to improve reliability and effectiveness.
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