期刊
CHEMBIOCHEM
卷 15, 期 8, 页码 1101-1105出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201400032
关键词
fluorescent probes; hydrolases; Mycobacterium tuberculosis; protein imaging; sulfatases
资金
- NIH postdoctoral fellowship [F32CA136109]
- Berkeley Fellowship for Graduate Study
- NIH [AI51622]
Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases.
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