期刊
CHEMBIOCHEM
卷 15, 期 2, 页码 209-212出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201300714
关键词
cross-coupling; Heck reaction; homogeneous catalysis; palladium; protein modifications
资金
- VIDI grant from the Netherlands Organization for Scientific Research (NWO) [016.122.302]
An increasing number of chemical reactions are being employed for bio-orthogonal ligation of detection labels to protein-bound functional groups. Several of these strategies, however, are limited in their application to pure proteins and are ineffective in complex biological samples such as cell lysates. Here we present the palladium-catalyzed oxidative Heck reaction as a new and robust bio-orthogonal strategy for linking functionalized arylboronic acids to protein-bound alkenes in high yields and with excellent chemoselectivity even in the presence of complex protein mixtures from living cells. Advantageously, this reaction proceeds under aerobic conditions, whereas most other metal-catalyzed reactions require inert atmosphere.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据