Molecularly imprinted polymer library on a microtiter plate. High-throughput synthesis and assessment of cinchona alkaloid-imprinted polymers

A molecularly imprinted polymer library was prepared on a 96-well microtiter plate, using a liquid-handing equipment that was programmed to prepare various breeds of cinchonidine-imprinted polymers. The resultant polymers, immobilized on the bottom surface of the wells, were screened for the binding capacity by fluorescence measurement, using a microplate reader. Comparison with a conventional assessment by FIA (flow injection analysis) demonstrated the reliability and the high-throughput performance of the microtiter plate-based combinatorial molecular imprinting.