期刊
CHEMBIOCHEM
卷 14, 期 4, 页码 457-466出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201200732
关键词
amino acid-specific isotope labeling; cell-free protein expression; NMR spectroscopy; protein ligation; segmental isotope labeling
资金
- ETH Zurich
- Swiss National Science Foundation through the National Center of Competence in Research (NCCR) Structural Biology (Iso lab)
- Swiss National Science Foundation [31003AB_133134]
- Swiss National Science Foundation (SNF) [31003AB_133134] Funding Source: Swiss National Science Foundation (SNF)
Current solution NMR techniques enable structural investigations of proteins in molecular particles with sizes up to several hundred kDa. However, the large molecular weight of proteins in such systems results in increased numbers of NMR signals, and the resulting spectral overlap typically imposes limitations. For multidomain proteins, segmental isotope labeling of individual domains facilitates the spectral interpretation by reducing the number of signals, but for large domains with small signal dispersion, signal overlap can persist. To overcome limitations arising from spectral overlap, we present a strategy that combines cell-free expression and ligation of the expressed proteins to produce multidomain proteins with selective amino acid-type labeling in individual domains. The bottleneck of intrinsically low cell-free expression yields of precursor molecules was overcome by introducing new fusion constructs that allowed milligram production of ligation-competent domains labeled in one or multiple amino acid types. Ligation-competent unlabeled partner domains were produced in vivo, and subsequent domain ligation was achieved by using an on-column strategy. This approach is illustrated with two multidomain RNA-binding proteins, that is, the two C-terminal RNA-recognition motifs of the human polypyrimidine tract-binding protein, and two highly homologous helixturnhelix domains of the human glutamyl-prolyl-tRNA synthetase.
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