期刊
CHEMBIOCHEM
卷 11, 期 12, 页码 1686-1691出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201000235
关键词
antibodies; carbohydrates; glycoconjugates; ligand density; vaccines
资金
- NIH, National Cancer Institute, Center for Cancer Research
Small peptides and oligosaccharides are important antigens for the development of vaccines and the production of monoclonal antibodies Because of their small size, peptides and oligosaccharides are non-immunogenic on their own and typically must be conjugated to a larger carrier protein to elicit an immune response. Selection of a suitable carrier protein, conjugation method, and hapten density are critical for generating an optimal immune response We used a glycan array to compare the repertoire of antibodies induced after immunizing with either low or high-density conjugates of the tumor-associated Tn antigen. At high hapten density, a broader range of antibodies was induced, and reactivity to the clustered Tn antigen was observed. In contrast, antibodies induced by the low-density conjugate had narrower reactivity and did not bind the clustered Tn antigen.
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