The controlled glycosylation of a protein with a bivalent glycan: towards a new class of glycoconjugates, glycodendriproteins

The use of a novel bivalent carbohydrate modification reagent, based on a flexible, branched divalent core in a combined site-directed mutagenesis and chemical modification strategy has allowed the first controlled synthesis of a pure protein bearing a branched glycan or a first generation glycodendriprotein.