期刊
CHEMBIOCHEM
卷 10, 期 17, 页码 2745-2752出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200900392
关键词
molecular evolution; native chemical ligation; RNA structures; RNA; template catalysis
资金
- Innovative Areas Emergence in Chemistry [27111518]
- Exploratory Research [19657071]
- MEXT Japan
- Grants-in-Aid for Scientific Research [19657071] Funding Source: KAKEN
The template effect plays important roles not only in modern synthetic and enzymatic catalysis but also in the ancient RNA polypeptide (RNP) world which has been postulated to be a crucial stage in the origin of life. To mimic primitive template catalysis of peptide ligations by RNAs, we previously reported the design and synthesis of a ternary RNP complex in which the ligation of two peptides was significantly facilitated by a template RNA with two peptide binding units. However, RNA molecules also promoted the ligation reaction in a nonspecific manner through electrostatic interactions between RNA and basic peptides. In this study, we suppressed this effect by reducing the length of the original template derived from the Tetrahymena intron RNA. This modification however decreased the template ability for the specific reaction. As an alternative RNA that was as effective as the original template, we found that a self dimerizing RNA was a promising template for peptide ligation without a nonspecific effect.
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