期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 67, 期 1, 页码 469-472出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.1.469-472.2001
关键词
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A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a mutational hot spot, and cloning of heteroduplexes and chimeras. These data may partially explain the high degree of microheterogeneity typical of sequence clusters detected in environmental clone libraries.
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