期刊
NEUROSCIENCE
卷 106, 期 2, 页码 287-301出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0306-4522(01)00278-0
关键词
glutamate; neurotoxicity; interneuron; phosphonate; System x(c)(-); beta-L-ODAP
资金
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS035608, R01NS035073] Funding Source: NIH RePORTER
- NINDS NIH HHS [NS 35608, NS 35073] Funding Source: Medline
A brief exposure of hippocampal slices to L-quisqualic acid sensitizes CAI pyramidal neurons 30-250-fold to depolarization by two classes of excitatory amino acid analogues: (1) those whose depolarizing effects are rapidly terminated following washout, e.g. L-2-amino-4-phosphonobutanoic acid (L-AP4) and L-2-amino-6-phosphonohexanoic acid (L-AP6) and (2) those whose depolarizing effects persist following washout, e.g. L-aspartate-beta -hydroxamate (L-A betaH). This process has been termed quisqualate sensitization. In this study we directly examine the role of amino acid transport systems in the induction of quisqualate sensitization. We report that L-quisqualate is a low-affinity substrate (K-M = 0.54 mM) for a high capacity (V-max = 0.9 nmol (mg protein)(-1) min(-1)) Na+-dependent transport system(s) and a high-affinity substrate (K-M = 0.033 mM) for a low-capacity (V-max = 0.051 nmol (mg protein)(-1) min(-1)) transporter with properties similar to the cystine/glutamate exchange carrier, System x(c)(-). We present evidence that suggests that System x(c)(-) participates in quisqualate sensitization. First, simultaneous application Of L-quisqualate and inhibitors of System x(c)(-) but not inhibitors of Na+-dependent glutamate transporters, prevents the subsequent sensitization of hippocampal neurons to phosphonates or L-A betaH. Second, L-quisqualic acid only sensitizes hippocampal neurons to other substrates of System x(c)(-) including cystine. Third, immunocytochemical analysis Of L-quisqualate uptake demonstrates that only inhibitors of System x(c)(-) inhibit the highly concentrative uptake of L-quisqualate into a widely dispersed group of GABAergic hippocampal interneurons. We conclude that quisqualate sensitization is a direct consequence of the unique interaction of various excitatory amino acids, namely L-quisqualate, cystine, and phosphonates, with the exchange carrier, System x(c)(-) Therefore, the results of this study have important implications for the mechanism by which L-quisqualate, and other substrates of this transporter which are also excitatory amino acid agonists, (such as glutamate and beta -N-oxalyl-L-alpha,beta -diaminopropionic acid, beta -L-ODAP) may trigger neurotoxicity. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.
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