期刊
NEUROSCIENCE
卷 104, 期 2, 页码 335-346出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0306-4522(01)00096-3
关键词
bistable state; prefrontal cortex; dopamine; excitability; dopamine agonists
资金
- NIMH NIH HHS [MH45156, MH1055, MH57440] Funding Source: Medline
- NATIONAL INSTITUTE OF MENTAL HEALTH [K02MH001055, R37MH057440, R01MH057440, P50MH045156] Funding Source: NIH RePORTER
Prefrontal cortex neurons recorded in vivo exhibit bistable activity states! consisting of a depolarized phase (-55 mV) and a hyperpolarized phase (-85 mV). These up and down states have durations ranging from 800 ms to 1 s and a periodicity of approximately 1 Hz. This study examines the state-dependency of prefrontal cortical neuron responses to dopamine, in which the bistable-state was approximated in vitro by intracellular current injection. At resting membrane potential (n = 10), dopamine caused a significant depolarization of the membrane potential without altering any of the other electrophysiological characteristics tested. In contrast, both dopamine (30 muM, 5 min) and the D1 receptor agonist SKF 38393 (5 and 10 muM) increased cell excitability when the cell was in the depolarized state (i.e., -55 mV) but not the hyperpolarized state (i.e., -85 mV: n = 10). This increase in excitability was accompanied by a decrease in the rheobase current. The SKF 38393-enhanced excitability was dose-dependent and could be blocked by bath administration of the D1 receptor antagonist SCH 23390 (5 and 10 muM) Administration of the GABA antagonist bicuculline (7 muM) plus the N-methyl-D-aspartate channel blocker CPP (10 muM) produced an additional increase in the excitability of prefrontal cortex neurons that was not dependent on the membrane potential. From these data we suggest that dopamine exerts state-dependent modulatory effects on the excitability of neurons in deep layers of the prefrontal cortex. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.
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