Comparison of immunoextraction sorbents prepared from monoclonal and polyclonal anti-isoproturon antibodies and optimization of the appropriate monoclonal antibody-based sorbent for environmental and biological applications

Adsorbents involving antigen-antibody interactions, so-called immunosorbents, have high affinity and class-selective extraction properties. Although most studies of immunosorbents are based on the use of polyclonal antibodies, to ensure a good repeatability with this kind of sorbent monoclonal antibodies are required. This study shows that the cross-reactivity of monoclonal anti-isoproturon antibodies is similar to that of polyclonal anti-isoproturon antibodies. To reduce the price of the resulting immunosorbent, the bonding density has been optimized. The choice of activated silica rather than Sepharose as solid support is discussed. The effect of sample volume and of the amount of immunosorbent on extraction recoveries for some phenylureas has been studied. It appears that the amount of immunosorbent can be readily adapted to the detection limit required.