期刊
CYTOGENETICS AND CELL GENETICS
卷 93, 期 1-2, 页码 77-82出版社
KARGER
DOI: 10.1159/000056953
关键词
-
资金
- NATIONAL CANCER INSTITUTE [P30CA034196] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P40RR001183] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM046697] Funding Source: NIH RePORTER
- NCI NIH HHS [CA34196] Funding Source: Medline
- NCRR NIH HHS [P40 RR01183] Funding Source: Medline
- NIGMS NIH HHS [GM46697] Funding Source: Medline
The paracentric inversion In(3)55Rk on mouse Chromosome 3 (Chr 3) was induced by cesium irradiation. Genetic crosses indicate the proximal breakpoint cosegregates with D3Mit324and D3Mit92; the distal breakpoint cosegregates with D3Mit127. D3Mit160, and D3Mit200. Giemsa-banded chromosomes show the inversion spans similar to 80% of Chr 3. The proximal breakpoint occurs within band 3A2, not 3B as reported previously; the distal breakpoint occurs within band 3H3. Mice homozygous for the inversion exhibit nephropathy indicative of uricase deficiency. Southern blot analyses of urate oxidase, Uox, show two RFLPs of genomic mutant DNA: an EcoRI site between exons 4-8 and a BamHI site 3' to exon 6. Mutant cDNA fails to amplify downstream of base 844 at the 3' end of exon 7. FISH analysis of chromosomes from inversion heterozygotes, using a cosmid clone containing genomic wild-type DNA for Uox exons 2-4, shows that a 5' segment of the mutated Uox allele on the inverted chromosome has been transposed from the distal breakpoint region to the proximal breakpoint region. Clinical, histopathological, and Northern analyses indicate that our radiation-induced mutation, uox(ln), is a putative null. Copyright (C) 2001 S. Karger AG, Basel.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据