期刊
NATURE BIOTECHNOLOGY
卷 19, 期 1, 页码 51-55出版社
NATURE AMERICA INC
DOI: 10.1038/83505
关键词
G-G mismatch; synthetic ligand; surface plasmon resonance; SNPs; mutation detection
Here we have designed and synthesized ligands that specifically bind with high affinity (K-d = 53 nM) to the guanine (G)-guanine mismatch, one of four types of single-nucleotide polymorphism (SNP). Detection of the G-G mismatch was performed by a surface plasmon resonance (SPR) assay using a sensor chip carrying the G-G specific ligand on its surface. The accuracy of the G-G mismatch detection by the SPR sensor was demonstrated by a marked SPR response obtained only for the DNA containing the G-G mismatch. DNAs containing G-A and G-T mismatches, as well as a fully matched duplex, produced only a weak response. Furthermore, this assay was found applicable for the detection of SNP existing in PCR amplification products of a 652-nucleotide sequence of the HSP70-2 gene.
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