期刊
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
卷 16, 期 12, 页码 1403-1408出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b106355n
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Cation and anion exchange HPLC were used to separate a mixture of 12 selenium species comprising selenoamino acids, selenonium ions and inorganic selenium. The cationic species were separated from each other and from the co-injected anions using a cation exchange column with gradient elution by aqueous pyridinium formate at pH similar to 3 as the mobile phase. The anionic species were separated using an anion exchange column with isocratic elution by an aqueous salicylate-TRIS mobile phase at pH 8.5. The separated selenium species were detected as Se-80 by ICP-dynamic reaction cell (DRC)-MS. The analytical methods were applied to the analysis of yeast and algae enriched in selenium. The yeast was treated with beta -glucosidase followed by a protease mixture for dissolution of the cell walls and selenium-containing peptides, respectively. The second to largest HPLC peak after that corresponding to selenomethionine was ascribed to selenomethionine-Se-oxide (SeOMet) by retention time matching with a SeOMet standard, which was characterised by HPLC-electrospray (ES)-MS. Se-methylselenocysteine was detected based on co-chromatography with the standard substance spiked to the yeast hydrolysate. A trichloroacetic acid extract of Chlorella algae contained dimethylselenonium propionate (DMSeP), which was verified by HPLC-ES-MS. Se-allylselenocysteine and selenoethionine was detected at the low ng g(-1) concentration level based on co-chromatography with the standard substances spiked to the algal extract.
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