期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 84, 期 -, 页码 58-63出版社
WILEY-LISS
DOI: 10.1002/jcb.10066
关键词
directed evolution; recombination; mutagenesis; computational methods
Directed evolution has proven to be a successful strategy for the modification of enzyme properties. To date, the preferred experimental procedure has been to apply mutations or crossovers randomly throughout the gene. With the emergence of powerful computational methods, it has become possible to develop focused combinatorial searches, guided by computer algorithms. Here, we describe several computational methods that have emerged to aid the optimization of mutant libraries, the targeting of specific residues for mutagenesis, and the design of recombination experiments. (C) 2002 Wiley-Liss, Inc.
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