期刊
JOURNAL OF BIOMECHANICS
卷 34, 期 1, 页码 59-65出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0021-9290(00)00158-5
关键词
chondrocytes; in vitro; mechanotransduction; fluid flow; shear stress; calcium
资金
- NIAMS NIH HHS [AR45989] Funding Source: Medline
- NIA NIH HHS [AG 15107] Funding Source: Medline
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR045989] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG015107] Funding Source: NIH RePORTER
Mechanical loading is a well-known regulator of cartilage metabolism. This suggests that a loading-induced physical signal regulates chondrocyte behavior. Previous studies have focused on the effects of steady fluid Row on chondrocytes. In contrast to steady Row, loading induced fluid flow occurs in an oscillatory pattern and includes a reversal of how direction with each loading event. In this study we examined the hypothesis that oscillating fluid flow increases cytosolic Ca2+ concentration([Ca2+](i))in bovine articular chondrocytes (BAC) in a frequency-dependent manner and that the presence of serum affects this response. The aims of our study were to examine(1) whether BAC respond to physiologic oscillating fluid flow in vitro and compare these results to steady fluid flow, (2) the effect of fetal bovine serum on fluid flow responsiveness of BAC and (3) whether the response of BAC to fluid flow is flow rate and/or frequency dependent. [Ca2+](i) was quantified using the fluorescent dye fura-2. BAC were exposed to steady, 0.5, 1, or 5 Hz sinusoidal oscillating fluid flow at five different flow rates in a parallel plate flow chamber. Our findings demonstrate that BAC respond to oscillating fluid now with an increase in [Ca2+](i) (p > 0.05), and furthermore, chondrocyte responsiveness to fluid flow increases with peak flow rate (p < 0.0001) and decreases with increasing frequencies do < 0.0001). Finally, the presence of serum in the media potentiated the responsiveness of BAC to fluid flow (p < 0.0001). Our results suggest an important role for mechanical load-induced oscillating fluid Row in chondrocyte mechanotransduction. (C) 2000 Elsevier Science Ltd. All rights reserved.
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