期刊
CEREBRAL CORTEX
卷 23, 期 8, 页码 1798-1810出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhs154
关键词
cerebral cortex; confocal; intracellular; Lucifer Yellow; 3D reconstructions
资金
- Ministerio de Economia y Competitividad (CSIC)
- Ministerio de Economia y Competitividad [SAF 2009-09394]
- CIBERNED [CB06/05/0066]
- Fundacion CIEN
- HHMI
- National Eye Institute
- Ministerio de Economia y Competitividad (Spanish partner of the Blue Brain Project initiative from EPFL)
Dendritic spines of pyramidal neurons are targets of most excitatory synapses in the cerebral cortex. Recent evidence suggests that the morphology of the dendritic spine could determine its synaptic strength and learning rules. However, unfortunately, there are scant data available regarding the detailed morphology of these structures for the human cerebral cortex. In the present study, we analyzed over 8900 individual dendritic spines that were completely 3D reconstructed along the length of apical and basal dendrites of layer III pyramidal neurons in the cingulate cortex of 2 male humans (aged 40 and 85 years old), using intracellular injections of Lucifer Yellow in fixed tissue. We assembled a large, quantitative database, which revealed a major reduction in spine densities in the aged case. Specifically, small and short spines of basal dendrites and long spines of apical dendrites were lost, regardless of the distance from the soma. Given the age difference between the cases, our results suggest selective alterations in spines with aging in humans and indicate that the spine volume and length are regulated by different biological mechanisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据