期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 31, 期 1, 页码 1-5出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0965-1748(00)00157-0
关键词
thioglucosides (glucosinolates); thioglucoside glucohydrolase (EC 3.2.3.1 myrosinase); Brevicoryne brassicae; aphid
Plant myrosinases and glucosinolates constitute a defence system in cruciferous plants towards pests and diseases. We have purified for the first time a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.) to homogeneity. The protein was N-terminally blocked and protease (trypsin and lys c) degradation gave peptides of which five were sequenced. The protein is a dimer with subunits of mass 54 kDa+/-500 Da. Western blot analysis with an anti-aphid myrosinase antibody showed a strong cross reaction with a protein extract from the Brassica specialist, B. brassicae. The anti-aphid myrosinase antibody does not cross react with plant myrosinase neither does an anti-plant myrosinase antibody cross react with aphid myrosinase. (C) 2001 Elsevier Science Ltd. All rights reserved.
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