期刊
CEREBRAL CORTEX
卷 22, 期 5, 页码 996-1006出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhr182
关键词
barrel cortex; cortical patterning; cytoarchitecture; ROR beta; thalamocortical innervation
资金
- National Institutes of Health (NIH) [NS49553, NS45523, NS41590]
- Harvard Stem Cell Institute
- Spastic Paraplegia Foundation
- Travis Roy Foundation
- Massachusetts Spinal Cord Injury Research Fund
- Jane and Lee Seidman Fund for CNS Research
- Emily and Robert Pearlstein Fund for Nervous System Repair
- Swiss National Science Foundation
- Pierre Mercier Science Foundation
- NIH predoctoral NRSA [F31 NS063516]
- Mary Gordon Roberts Fellowship
- Caja Madrid Foundation
Neurons in layer IV of the rodent whisker somatosensory cortex are tangentially organized in periodic clusters called barrels, each of which is innervated by thalamocortical axons transmitting sensory information from a single principal whisker, together forming a somatotopic map of the whisker pad. Proper thalamocortical innervation is critical for barrel formation during development, but the molecular mechanisms controlling layer IV neuron clustering are unknown. Here, we investigate the role in this mapping of the nuclear orphan receptor ROR beta, which is expressed in neurons in layer IV during corticogenesis. We find that ROR beta protein expression specifically increases in the whisker barrel cortex during barrel formation and that in vivo overexpression of ROR beta is sufficient to induce periodic barrel-like clustering of cortical neurons. Remarkably, this clustering can be induced as early as E18, prior to innervation by thalamocortical afferents and whisker derived-input. At later developmental stages, these ectopic neuronal clusters are specifically innervated by thalamocortical axons, demonstrated by anterograde labeling from the thalamus and by expression of thalamocortical-specific synaptic markers. Together, these data indicate that ROR beta expression levels control cytoarchitectural patterning of neocortical neurons during development, a critical process for the topographical mapping of whisker input onto the cortical surface.
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