Bacterial replication initiator DnaA. Rules for DnaA binding and roles of DnaA in origin unwinding and helicase loading

We review the processes leading to the structural modifications required for the initiation of replication in Escherichia coli, the conversion of the initial complex to the open complex, loading of helicase, and the assembly of two replication forks. Rules for the binding of DnaA to its binding sites are derived, and the properties of ATP-DnaA are described. We provide new data on cooperative interaction and dimerization of DnaA proteins of E. coli. Streptomyces and Thermus thermophilus. and on the stoichiometry of DnaA-oriC complexes of E. coli. (C) 2001 Societe francaise de biochimie et biologie moleculaire/ Editions scientifiques et medicales Elsevier SAS.