期刊
CEREBRAL CORTEX
卷 21, 期 4, 页码 806-820出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhq154
关键词
ATP-induced signaling; brain slice; cortical astroglial cells; immunohistochemistry; P2X(7) receptors; whole-cell patch-clamp
资金
- Deutsche Forschungsgemeinschaft (DFG) [IL 20/16-1, IL 20/19-1, FR 1253/3-1]
- VW-Foundation [I/82 940]
- German Federal Ministry of Education and Research (BMBF) [PtJ-Bio 0313909]
ATP is an important neuronal and astroglial signaling molecule in the brain. In the present study, brain slices were prepared from the prefrontal cortex (PFC) of Wistar rats and 2 lines of mice. P2X(7) receptor immunoreactivity was colocalized with astro- and microglial but not neuronal markers. Whole-cell patch-clamp recordings showed that, in astroglial cells, dibenzoyl-ATP (BzATP) and ATP caused inward currents, near the resting membrane potential. The inactivity of alpha,beta-methylene ATP, as well as the potency increases of BzATP and ATP in a low divalent cation (X2+)-containing superfusion medium suggested the involvement of P2X(7) receptors. This idea was corroborated by the inhibition of these current responses by PPADS, Brilliant Blue G, A 438079, and calmidazolium. Ivermectin, trinitrophenyl-adenosine-5'-triphosphate, and cyclopentyl-dipropylxanthine did not alter the agonist effects. The reversal potential of BzATP was near 0 mV, indicating the opening of cationic receptor channels. In a low X2+ superfusion medium, ATP-induced current responses in PFC astroglial cells of wild-type mice but not of the P2X(7) knockouts. Hence, cortical astroglia of rats and mice possess functional P2X(7) receptors. These receptors may participate in necrotic/apoptotic or proliferative reactions after stimulation by large quantities of ATP released by central nervous system injury.
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