期刊
CEREBELLUM
卷 13, 期 3, 页码 307-317出版社
SPRINGER
DOI: 10.1007/s12311-014-0548-5
关键词
Purkinje; Development; Gene expression; Axon guidance cues; Cell sorting
资金
- Human Frontier Science Program Organization [CDA-0015-2006-C]
- INSERM-AVENIR program
- Foundation Fyssen
- Federation pour la recherche sur le cerveau (FRC)
- Fondation pour la recherche Medicale (FRM)
- ANR [ANR-08-JCJC-0044]
- Agence Nationale de la Recherche (ANR) [ANR-08-JCJC-0044] Funding Source: Agence Nationale de la Recherche (ANR)
The establishment of precise neural circuits during development involves a variety of contact-mediated and secreted guidance molecules that are expressed in a complementary fashion by different cell types. To build a functional circuit, each cell type must first trigger an intrinsic genetic program that is led by their environment at a key time point. It is therefore essential to identify the different cell-specific and stage-specific transcriptional profiles expressed by neurons. However, very few studies have been done to address this issue thus far. Herein, we have carried out a large-scale quantitative real-time PCR analysis of all classical axon guidance molecules (i.e., Semaphorins, Netrins, Ephrins, and Slits) and their receptors expressed by Purkinje cells (PCs) at specific stages of postnatal cerebellar development in vivo. Most cerebellar connections are setup in a well-characterized sequential manner during postnatal development and lead to the fine regulation of the PC, the sole output of the structure. Our analysis of the relative expression of these guidance cues has uncovered a dynamic expression pattern corresponding to specific stages of cerebellar development, thus providing a starting point for studying the role of these axon guidance molecules in cerebellar wiring.
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