Fusarium langsethiae on kernels of winter wheat in Poland - Occurrence and mycotoxigenic abilities

The object of the study was mycological analysis of settlement of winter wheat grain samples collected in 2005/2006 and 2006/2007 cropping seasons with the special respect to a new species - Fusarium langsethiae. Its presence was noted in 38 samples in relatively low intensity (from 0.5 up to 3.5%). PCR assay confirmed identity of 38 isolates as F langsethiae. Mycelium of tested strains of F. langsethiae was whitish with powdery appearance. Microconidia were formed napiform or globose, nonseptate, formed in heads. Macroconidia, sclerotia, and chlamydospores were not present after 3 weeks of incubation. Growth rates ranged from 5.4 to 10.3 mm/day. No isolate had potential ability to type B trichothecenes. Detailed chemical analyses of two strains showed them as producers of T-2 and HT-2 toxins at high concentrations.