期刊
CEREAL RESEARCH COMMUNICATIONS
卷 36, 期 -, 页码 415-419出版社
CEREAL RES INST
DOI: 10.1556/CRC.36.2008.Suppl.B.34
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Transposon mutagenesis was applied to generate Mutants in Fusarium graminearum. The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant Could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system it powerful tool in the functional analyses of the > 10,000 genes predicted in the F graminearum genome.
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