期刊
PROSTATE
卷 50, 期 1, 页码 46-53出版社
WILEY-LISS
DOI: 10.1002/pros.10031
关键词
epithelium; clonogenic; cytokeratins; bcl-2; differentiation
资金
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R21DK059177] Funding Source: NIH RePORTER
- NIDDK NIH HHS [DK59177] Funding Source: Medline
BACKGROUND. Primary cultures of mouse prostatic epithelial cells were studied to identify cells having stem cell characteristics. METHODS. Culture conditions supporting the growth of mouse prostatic epithelial cells were identified. Immunostaining identified cells expressing cytokeratin 5 (K5), cytokeratin 8 (K8), and bcl-2. Cells with clonogenic capacity were identified by replating dissociated cells from primary colonies. RESULTS. Only 2-4% of prostatic epithelial cells that were plated formed colonies. Most cells cultured for 2-4 days expressed bcl-2, K5, and KS, After 6 days in culture, colonies had two distinct phenotypes, star- or disc-shaped. In colonies of both phenotypes, only a few cells expressed K5 and bcl-2, while all cells expressed K8. Upon replating, two-thirds of star-shaped colonies gave rise to a single colony; of 29 disc-shaped colonies, none gave rise to new colonies, CONCLUSIONS. Colonies deriving from putative prostatic epithelial stem cells are identified. These cells express K5 and bcl-2 and a-re clonogenic. (C) 2002 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据