期刊
CERAMICS INTERNATIONAL
卷 39, 期 6, 页码 6145-6152出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceramint.2013.01.033
关键词
Fluoro-hydroxyapatite; Protein adsorption; Bioactivity; Osteoblast
资金
- Iranian Pasteur Institute
Phase pure (HAp) and fluoride-substituted apatite (FHAp) powders were synthesized by the hydrothermal method and their specific surface area was determined by atomic force microscopy (AFM). Human osteoblast cells were used to evaluate the bioactivity of the samples. The influence of fluoride ions on the alteration of cell medium components was analyzed using UV-visible spectroscopy in different exposure times. The protein interaction behavior with the pure and substituted powders was assessed by the Bradford method and SDS-PAGE analysis. The mRNA expression level was detected by real time PCR and it was observed that the presence of an optimal level of fluoride ions in the apatite structure has an inductive effect on collagen I and alkaline phosphatase (ALP) expression levels. A similar trend was also observed in the protein adsorption profile indicating that the fluoride content in the apatite lattice has a significant impact on controlling its biological performance. The maximum protein adsorption was observed on the sample with the chemical composition of Ca-10(PO4)(6)(OH)(1.33)F-0.67. (C) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据