期刊
PHYTOCHEMICAL ANALYSIS
卷 13, 期 2, 页码 79-86出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/pca.627
关键词
H-1-NMR; C-13-NMR; HPLC-PAD; HPLC-MS; 3,5-O-digalloyl quinic acid; 3,4,5-O-trigalloyl quinic acid; myricetin glucuronide; delphinidin 3-O-glucoside
Separation, identification and quantification of polyphenols was carried out on leaves of Pistacia lentiscus L., an evergreen member of the family Anacardiaceae, using semi-preparative HPLC, HPLC-photodiode array detection and HPLC-MS analysis, together with H-1- and C-13 NMR. Three major classes of secondary metabolites were detected: (i) gallic acid and galloyl derivatives of both glucose and quinic acid; (ii) flavonol glycosides, i.e. myricetin and quercetin glycosides; and (iii) anthocyanins, namely delphinidin 3-O-glucoside and cyanidin 3-O-glucoside. Low amounts of catechin were also detected. The concentration of galloyl derivatives was extremely high, representing 5.3% of the leaf dry weight, and appreciable amounts of myricetin derivatives were also detected (1.5% on a dry weight basis). These findings may be useful in establishing a relationship between the chemical composition of the leaf extract and the previously reported biological activity of P. lentiscus, and may also assign a new potential role of P. lentiscus tissue extracts in human health care. Copyright (C) 2002 John Wiley Sons, Ltd.
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