Differentiation of three varieties of Rhizoctonia circinata; var. circinata, var. oryae and var. zeae on the basis of cellular fatty acid composition

Cellular fatty acid analysis was employed to differentiate three varieties of Rhizoctonia circinata; var. circinata, var. oryzae and var. zeae. Eight fatty acids including myristic (14 : 0), pentadecanoic (15 : 0), palmitic (16 : 0), palmitoleic (16 : 1 cis9), stearic (18 : 0), oleic (18 : 1 cis9), linoleic(18 2 cis9,12) and linolenic (18 : 3 cis9,12)acids were present in isolates of all three varieties of R. circinata. Heptadecanoic acid (17 : 0) was detected in isolates of R. circinata var. zeae but not in isolates of R. circinata var. circinata or R. circinata var. oryzae. Palmitic, oleic and linoleic acids were the major fatty acids found, comprising 94-98% of the whole-cell fatty acid content. The remaining fatty acids were present in small amounts. Based on the composition (%) of fatty acids, isolates of R. circinata var. circinata, R. circinata var. oryzae and R. circinata var. zeae were clearly differentiated into three groups as shown by principal component and cluster analyses. This finding agrees well with the grouping of R. circinata into three varieties based on differences in colony morphology of the vegetative state. In principal component and cluster analysis, isolates of R. circinata var. circinata from Japan and Alaska were indistinguishable.