期刊
JOURNAL OF IMMUNOLOGY
卷 168, 期 1, 页码 44-50出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.168.1.44
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资金
- NIAID NIH HHS [R01 AI 32972] Funding Source: Medline
- NIGMS NIH HHS [R01 GM 62995] Funding Source: Medline
- PHS HHS [R37 25032] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI032972] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM062995] Funding Source: NIH RePORTER
Microbial products such as LPS stimulate macrophages to produce a wide diversity of inducible gene products needed for immediate host defense and priming of an appropriate acquired immune response. In this study, we have examined LPS-inducible gene expression in subclones of a mouse macrophage cell line, RAW264, using cDNA microarrays. Even archetypal target genes such as TNF-alpha were not induced in all subclones, and there was no absolute correlation between expression of pairs of genes. Nevertheless, the array analysis revealed clusters of genes that were more likely, to be coexpressed. RAW264 cells stably transfected with luciferase reporter genes driven by LPS-responsive promoters revealed the same kind of clonal heterogeneity. The results indicate that each LPS-inducible gene has its own inherent probability of activation in response to LPS.
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