期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 36, 期 5, 页码 869-876出版社
AMER CHEMICAL SOC
DOI: 10.1021/es011090q
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资金
- NIEHS NIH HHS [2P42ES04911-05] Funding Source: Medline
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P42ES004911] Funding Source: NIH RePORTER
The effects of chrysene and the ozonated byproducts on in vitro gap junctional intercellular communication (GJIC) were evaluated using the scrape loading/dye transfer (SL/DT) technique. A 1 mM solution of chrysene was ozonated at dosages of 1.75, 3, 4.25, and 5 mol O-3/mol chrysene (Chr). The early ozonation mixture, 1.75 mol O-3/mol Chr, exhibited greater inhibition to GJIC than chrysene and irreversible damage to cells leading to cell death. To determine the compounds potentially responsible for the increase in toxicity, the byproducts formed upon treatment with 1.44 mol O-3/mol Chr were separated into 14 fractions using RP-HPLC. The major compounds identified in the fractions were 2-(2'-formyl) phenyl-l-naphthaldehyde, 2-(2'formyl) phenyl-1-naphthoic acid, and 2-2-carboxyphenyl-1-naphthoic acid. 2-(2'-Formyl) phenyl-l-naphthaldehyde was determined to be the compound causing GJIC inhibition in sample fractions and byproduct mixtures.
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