Molecularly imprinted polymer coatings were synthesized in fused-silica capillary columns by the use of a surface-coupled radical initiator. The coatings were prepared using either toluene, dichloromethane, or acetonitrile in the prepolymerization mixtures and were 0.15-2 mum thick as determined by scanning electron microscopy. Solvent-dependent differences in appearance were observed. All the molecularly imprinted polymer-based open-tubular capillary columns were able to separate the enantiomers of propranolol by means of electrochromatography. Electrochromatographic performance was found to be dependent on the type of solvent used during the synthesis.
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